Article
Mutation of the Gs protein alpha subunit NH2 terminus relieves an attenuator function, resulting in constitutive adenylyl cyclase stimulation.
Molecular and cellular biology - 1 Jun 1990
Osawa S, Heasley L E, Dhanasekaran N, Gupta S K, Woon C W, Berlot C, Johnson G L
Abstract excerpt
G-proteins couple hormonal activation of receptors to the regulation of specific enzymes and ion channels. Gs and Gi are G-proteins which regulate the stimulation and inhibition, respectively, of adenylyl cyclase. We have constructed two chimeric cDNAs in which different lengths of the alpha subu...
Topics
- 1-Methyl-3-isobutylxanthine
- Adenylyl Cyclases
- Animals
- Cell Line
- Cell Membrane
- Chimera
- Cyclic AMP
- DNA
- Enzyme Activation
- GTP-Binding Proteins
- Isoproterenol
- Kinetics
- Macromolecular Substances
- Models, Structural
- Mutation