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Article

A mutation in the putative Mg(2+)-binding site of Gs alpha prevents its activation by receptors.

Molecular and cellular biology - 1 Oct 1991

Hildebrandt J D, Day R, Farnsworth C L, Feig L A

Abstract excerpt

The properties of a Gs alpha mutant with an Asn substituted for Ser at position 54, designated mutant 54Asn alpha s, were studied after expression in S49 alpha s-deficient (cyc-) cells. Ser-54 in alpha s is comparable to Ser-17 in Ras, which is involved in binding Mg2+ associated with bound nucleotide. 54Asn alpha s did not restore either hormone-induced cyclic AMP production in intact cyc- cells or...

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Identifiers

PMID: 1656212
DOI: 10.1128/mcb.11.10.4830-4838.1991
PMCID: PMC361450

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