Article
A mutation in the putative Mg(2+)-binding site of Gs alpha prevents its activation by receptors.
Molecular and cellular biology - 1 Oct 1991
Hildebrandt J D, Day R, Farnsworth C L, Feig L A
Abstract excerpt
The properties of a Gs alpha mutant with an Asn substituted for Ser at position 54, designated mutant 54Asn alpha s, were studied after expression in S49 alpha s-deficient (cyc-) cells. Ser-54 in alpha s is comparable to Ser-17 in Ras, which is involved in binding Mg2+ associated with bound nucle...
Topics
- Adenylyl Cyclases
- Animals
- Asparagine
- Binding Sites
- Blotting, Northern
- Cyclic AMP
- Enzyme Activation
- GTP-Binding Proteins
- Gene Expression Regulation, Enzymologic
- Guanosine 5'-O-(3-Thiotriphosphate)
- Guanosine Triphosphate
- Isoproterenol
- Kinetics
- Magnesium
- Mice
- Mutation
- Oncogene Protein p21(ras)
- Serine