A dominant negative G alpha s mutant is rescued by secondary mutation of the alpha chain amino terminus.

Article

A dominant negative G alpha s mutant is rescued by secondary mutation of the alpha chain amino terminus.

The Journal of biological chemistry - 15 Mar 1991

Osawa S, Johnson G L

Abstract excerpt

The Gs protein alpha subunit, alpha s, stimulates the activity of adenylyl cyclase. The sequence 223Asp-Val-Gly-Gly-Gln227 in the alpha s polypeptide is predicted to interact with the gamma-phosphate of GTP and mediate the conformational change involved in alpha s activation. Mutation of the alph...

Topics

Adenylyl Cyclases
Cell Line
Chimera
Cyclic AMP
Electrophoresis, Polyacrylamide Gel
Enzyme Activation
GTP-Binding Proteins
Mutation
Phenotype
Receptors, Adrenergic, beta

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