Article
mRNA destabilization triggered by premature translational termination depends on at least three cis-acting sequence elements and one trans-acting factor.
Genes & development - 1 Sept 1993
Peltz S W, Brown A H, Jacobson A
Abstract excerpt
Nonsense mutations in a gene can accelerate the decay rate of the mRNA transcribed from that gene, a phenomenon we describe as nonsense-mediated mRNA decay. Using amber (UAG) mutants of the yeast PGK1 gene as a model system, we find that nonsense-mediated mRNA decay is position dependent, that is, nonsense mutations within the initial two-thirds of the PGK1-coding region accelerate the decay rate of the PGK1...
Topics
- Base Sequence
- Codon
- DNA, Fungal
- Fungal Proteins
- Molecular Sequence Data
- Mutation
- Phosphoglycerate Kinase
- Protein Biosynthesis
- RNA Helicases
- RNA, Messenger
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Terminator Regions, Genetic
- Trans-Activators
- Transcription Factors