Analysis of large X-ray-induced mutant populations by denaturing gradient gel electrophoresis.

Article

Analysis of large X-ray-induced mutant populations by denaturing gradient gel electrophoresis.

Radiation research - 1 Aug 1993

Okinaka R T, Anzick S L, Oller A, Thilly W G

Abstract excerpt

To increase the precision by which predominant point mutations can be observed, hypoxanthine guanine phosphoribosyl transferase (HPRT)-deficient mutants selected en masse from large X-irradiated cultures of human lymphoblastoid cells (line TK6) were analyzed by denaturing gradient gel electrophor...

Topics

Base Sequence
Cell Line
DNA, Single-Stranded
Electrophoresis, Polyacrylamide Gel
Exons
Gene Amplification
Humans
Hypoxanthine Phosphoribosyltransferase
Lymphocytes
Male
Molecular Sequence Data
Mutation
Polymerase Chain Reaction

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