Non-radioactive mismatch analysis to detect small mutations in human hypoxanthine-guanine phosphoribosyl transferase cDNA.

doi:10.7883/yoken1952.48.163

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Article

Non-radioactive mismatch analysis to detect small mutations in human hypoxanthine-guanine phosphoribosyl transferase cDNA.

Japanese journal of medical science & biology - 1 Aug 1995

Tsuboi K, Nose T, Okinaka R T, Chen D J

Abstract excerpt

We have combined a cDNA-driven PCR technique and a non-radioactive chemical-cleavage mismatch method, followed by a direct sequencing for detecting small mutations in the human hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene. HPRT cDNA was synthesized by RT-PCR from 1,000 wild-type or...

Topics

Base Sequence
Cells, Cultured
DNA, Complementary
Humans
Hypoxanthine Phosphoribosyltransferase
Molecular Sequence Data
Mutation
Polymerase Chain Reaction

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