Article
Screening for mutations in human HPRT cDNA using the polymerase chain reaction (PCR) in combination with constant denaturant gel electrophoresis (CDGE).
Mutation research - 1 Sept 1992
Smith-Sørensen B, Hovig E, Andersson B, Børresen A L
Abstract excerpt
Previously, we reported the modification of denaturing gradient gel electrophoresis called constant denaturant gel electrophoresis (CDGE). CDGE separates mutant fragments in specific melting domains. CDGE seems to be a useful tool in mutation detection. Since the hypoxanthine phosphoribosyltransf...
Topics
- Base Sequence
- Cloning, Molecular
- DNA
- Electrophoresis
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Molecular Sequence Data
- Mutation
- Nucleic Acid Denaturation
- Polymerase Chain Reaction
- Temperature