Article
Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR.
Nucleic acids research - 1 Nov 2003
Kermekchiev Milko B, Tzekov Anatoly, Barnes Wayne M
Abstract excerpt
Although the thermophilic bacterium Thermus aquaticus grows optimally at 70 degrees C and cannot grow at moderate temperatures, its DNA polymerase I has significant activity at 20-37 degrees C. This activity is a bane to some PCRs, since it catalyzes non-specific priming. We report mutations of Klentaq (an N-terminal deletion variant) DNA polymerase that have markedly reduced activity at 37 degrees C yet retain...
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