Article
Translesion synthesis across the (6-4) photoproduct and its Dewar valence isomer by the Y-family and engineered DNA polymerases.
Nucleic acids symposium series (2004) - 1 Jan 2008
Yamamoto Junpei, Loakes David, Masutani Chikahide, Simmyo Shizu, Urabe Kumiko, Hanaoka Fumio, Holliger Philipp, Iwai Shigenori
Abstract excerpt
We analyzed the translesion synthesis across the UV-induced lesions, the (6-4) photoproduct and its Dewar valence isomer, by using human DNA polymerases eta and iota in vitro. The primer extension experiments revealed that pol eta tended to incorporate dG opposite the 3' component of both lesions, but the incorporation efficiency for the Dewar isomer was higher than that for the (6-4) photoproduct. On the other...
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