Article
The implications of using mutagenic primers in combination with Taq polymerase having proofreading activity.
Biologicals : journal of the International Association of Biological Standardization - 1 Jun 2004
Papadopoulou E, Metaxa-Mariatou V, Hatzaki A, Hatzis Th, Nasioulas G
Abstract excerpt
Polymerases with proofreading activity provide high fidelity PCR amplifications. In this study we examined the consequences of using a Taq polymerase with proofreading activity, such as Optimase Taq polymerase, in combination with 4 different mutagenic reverse primers for the amplification of a 345-bp FII PCR product. The amplifications were performed with Optimase Taq polymerase (Transgenomic), and Taq DNA...
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