Article
Subtraction cloning of H-rev107, a gene specifically expressed in H-ras resistant fibroblasts.
Oncogene - 1 Feb 1994
Hajnal A, Klemenz R, Schäfer R
Abstract excerpt
We have isolated by subtractive hybridization a novel gene, called H-rev107, which is specifically expressed in a phenotypic revertant of H-ras transformed 208F rat fibroblasts. Apart from oncogene revertants, strong expression of H-rev107 was found in REF52 and EK-3 cells, two fibroblast lines resistant to transformation by activated H-ras oncogenes. In contrast, transformation-sensitive fibroblasts like 208F or...
Topics
- Adenovirus E1A Proteins
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Western
- Cell Line
- Cell Membrane
- Cell Transformation, Neoplastic
- Cloning, Molecular
- Cytoplasm
- DNA
- Down-Regulation
- Fibroblasts
- Genes, ras
- Molecular Sequence Data
- Molecular Weight
- Phenotype