Article
Isolation of flat revertants from human papillomavirus type 18 E6E7 transformed 3Y1 cells by transfection with a rat embryo fibroblast cDNA expression library.
Cell structure and function - 1 Dec 1993
Nakanishi K, Yong-Il H, Ishiwatari H, Takami Y, Hayasaka N, Yutsudo M, Nojima H, Hakura A
Abstract excerpt
A rat embryo fibroblast (REF) cDNA expression library was transfected into 3Y1 cells transformed by human papillomavirus type 18 E6 and E7 genes and 10 flat revertants were isolated. These revertants expressed the same levels of E6 and E7 mRNA as the parent cells, but had greatly reduced ability to form colonies in soft agar. Suppression of transformation was dominant in cell hybrids generated by fusing each...
Topics
- Animals
- Cell Separation
- Cell Survival
- Cell Transformation, Viral
- DNA, Complementary
- Embryo, Mammalian
- Fibroblasts
- Gene Library
- Genes, Tumor Suppressor
- Genetic Vectors