Article
Identification of residues critical for Ras(17N) growth-inhibitory phenotype and for Ras interaction with guanine nucleotide exchange factors.
Molecular and cellular biology - 1 Feb 1994
Quilliam L A, Kato K, Rabun K M, Hisaka M M, Huff S Y, Campbell-Burk S, Der C J
Abstract excerpt
The Ras(17N) dominant negative antagonizes endogenous Ras function by forming stable, inactive complexes with Ras guanine nucleotide exchange factors (GEFs; e.g., SOS1). We have used the growth-inhibitory phenotype of Ras(17N) to characterize two aspects of Ras interaction with GEFs. First, we us...
Topics
- 3T3 Cells
- Amino Acid Sequence
- Animals
- Cell Division
- Chloramphenicol O-Acetyltransferase
- Cysteine
- GTP-Binding Proteins
- Gene Expression
- Humans
- Mice
- Molecular Sequence Data
- Mutagenesis
- Myristic Acid
- Myristic Acids