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Article

Dominant inhibitory mutations in the Mg(2+)-binding site of RasH prevent its activation by GTP.

Molecular and cellular biology - 1 Oct 1991

Farnsworth C L, Feig L A

Abstract excerpt

We have previously demonstrated that substitution of Asn for Ser at position 17 of RasH yields a dominant inhibitory protein whose expression in cells interferes with endogenous Ras function (L. A. Feig, and G. M. Cooper, Mol. Cell. Biol. 8:3235-3243, 1988). Subsequent structural studies have shown that the hydroxyl group of Ser-17 contributes to the binding of Mg2+ associated with bound nucleotide. In this...

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Identifiers

PMID: 1922022
DOI: 10.1128/mcb.11.10.4822-4829.1991
PMCID: PMC361448

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