Article
Biological activity of human N-ras and K-ras genes containing the Asn17 dominant negative mutation.
Oncology research - 1 Jan 1994
Kaplan J B
Abstract excerpt
Substitution of asparagine for serine at position 17 of human H-ras results in an impaired GTP-binding activity, causing the mutant Ras protein to be locked in a constitutively inactive GDP-bound state. Expression of this mutant in NIH 3T3 cells inhibits cell proliferation by blocking endogenous...
Topics
- 3T3 Cells
- Alleles
- Amino Acid Sequence
- Animals
- Asparagine
- Cell Line, Transformed
- Codon
- Colony-Forming Units Assay
- Genes, Dominant
- Genes, ras
- Genetic Complementation Test
- Humans
- Mice
- Molecular Sequence Data
- Oncogene Proteins v-raf
- Plasmids
- Point Mutation
- Promoter Regions, Genetic