Article
Altering the antibody repertoire via transgene homologous recombination: evidence for global and clone-autonomous regulation of antigen-driven B cell differentiation.
The Journal of experimental medicine - 1 Jan 1995
Vora K A, Manser T
Abstract excerpt
Antibody VH transgenes containing small amounts of natural 5' and 3' flanking DNA undergo nonreciprocal homologous recombination with the endogenous Igh locus in B cells. The resulting "hybrid" heavy chain loci are generated at a low frequency but are fully functional, undergoing somatic hypermut...
Topics
- Animals
- Antibody Affinity
- Antibody Diversity
- Antigens
- B-Lymphocytes
- Base Sequence
- Cell Differentiation
- Genes, Immunoglobulin
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Mutation
- Recombination, Genetic
- p-Azobenzenearsonate