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Article

Isolation and characterization of novel mutations in the pSC101 origin that increase copy number.

Scientific reports - 25 Jan 2018

Thompson Mitchell G, Sedaghatian Nima, Barajas Jesus F, Wehrs Maren, Bailey Constance B, Kaplan Nurgul, Hillson Nathan J, Mukhopadhyay Aindrila, Keasling Jay D

Abstract excerpt

pSC101 is a narrow host range, low-copy plasmid commonly used for genetically manipulating Escherichia coli. As a byproduct of a genetic screen for a more sensitive lactam biosensor, we identified multiple novel mutations that increase the copy number of plasmids with the pSC101 origin. All mutations identified in this study occurred on plasmids which also contained at least one mutation localized to the RepA...

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Identifiers

PMID: 29371642
DOI: 10.1038/s41598-018-20016-w
PMCID: PMC5785507

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Source receipt: pubmed, retrieved 7 Sept 2026.

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