Article
Mutations that alter the third cytoplasmic loop of the a-factor receptor lead to a constitutive and hypersensitive phenotype.
Proceedings of the National Academy of Sciences of the United States of America - 1 Nov 1993
Boone C, Davis N G, Sprague G F
Abstract excerpt
The STE3 gene of Saccharomyces cerevisiae encodes a G protein-coupled receptor that is specific for the mating pheromone a-factor. The ste3L194Q mutation, which leads to the substitution of glutamine for leucine-194 within the third cytoplasmic loop of the receptor, resulted in a 20-fold increase in pheromone sensitivity and also caused partial constitutive activation of the response pathway. Moreover, other...
Topics
- Alleles
- Amino Acid Sequence
- Genes, Fungal
- Kinetics
- Mating Factor
- Molecular Sequence Data
- Mutagenesis
- Peptides
- Phenotype
- Pheromones
- Point Mutation