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Article

Strategies for isolating constitutively active and dominant-negative pheromone receptor mutants in yeast.

Methods in enzymology - 1 Jan 2010

Dosil Mercedes, Konopka James B

Abstract excerpt

Mating pheromone receptors of the yeast Saccharomyces cerevisiae are useful models for the study of G protein-coupled receptors. The mating pheromone receptors, Ste2 and Ste3, are not essential for viability so they can be readily targeted for analysis by a variety of genetic approaches. This chapter will describe methods for identification of two kinds of mutants that have been very informative about the...

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Identifiers

PMID: 21050926
DOI: 10.1016/B978-0-12-381296-4.00019-1
PMCID: PMC3741071

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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