Article
The cytoplasmic end of transmembrane domain 3 regulates the activity of the Saccharomyces cerevisiae G-protein-coupled alpha-factor receptor.
Genetics - 1 Feb 2002
Parrish William, Eilers Markus, Ying Weiwen, Konopka James B
Abstract excerpt
The binding of alpha-factor to its receptor (Ste2p) activates a G-protein-signaling pathway leading to conjugation of MATa cells of the budding yeast S. cerevisiae. We conducted a genetic screen to identify constitutively activating mutations in the N-terminal region of the alpha-factor receptor that includes transmembrane domains 1-5. This approach identified 12 unique constitutively activating mutations, the...
Topics
- GTP-Binding Proteins
- Models, Molecular
- Mutation
- Protein Conformation
- Protein Structure, Tertiary
- Receptors, Mating Factor
- Receptors, Peptide
- Saccharomyces cerevisiae
- Signal Transduction
- Structure-Activity Relationship
- Transcription Factors