Article
Complete subtyping of the HLA-A locus by sequence-specific amplification followed by direct sequencing or single-strand conformation polymorphism analysis.
Tissue antigens - 1 Aug 1995
Blasczyk R, Hahn U, Wehling J, Huhn D, Salama A
Abstract excerpt
A variety of reasons related to the HLA class I system has complicated the application of molecular approaches to HLA class I typing. Here we present a PCR-based HLA-A typing strategy considering the sequence variations of the two most polymorphic exons which allows complete subtyping of the HLA-...
Topics
- Alleles
- Base Sequence
- Gene Amplification
- HLA-A Antigens
- HLA-A1 Antigen
- HLA-A11 Antigen
- HLA-A2 Antigen
- HLA-A3 Antigen
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
