Article
HLA-DRB1*04 high resolution typing.
Experimental and clinical immunogenetics - 1 Jan 1996
Bouwens-Rombouts A G, Verduyn W, Versluis L F, Schreuder G M, Tilanus M G, Giphart M
Abstract excerpt
High resolution typing for HLA-DR4 is required to identify the individual subtypes. In this study a panel of DR4-positive samples was typed by both sequencing-based typing (SBT) and oligohybridization (PCR sequence-specific oligonucleotide; PCR-SSO). SBT reveals the highest resolution; moreover, ambiguous DRB1*04 allelic combinations can be resolved by a selective amplification of the individual alleles and...
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