Article
Cloning and characterization of a 3-methyladenine DNA glycosylase cDNA from human cells whose gene maps to chromosome 16.
Proceedings of the National Academy of Sciences of the United States of America - 15 Oct 1991
Samson L, Derfler B, Boosalis M, Call K
Abstract excerpt
We described previously the isolation of a Saccharomyces cerevisiae 3-methyladenine (3-MeAde) DNA glycosylase repair gene (MAG) by its expression in glycosylase-deficient Escherichia coli alkA tag mutant cells and its ability to rescue these cells from the toxic effects of alkylating agents. Here...
Topics
- Alkylating Agents
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Cell Death
- Chromosome Mapping
- Chromosomes, Human, Pair 16
- Cloning, Molecular
- Cricetinae
- DNA
- DNA Glycosylases
- DNA Repair
- Escherichia coli
- Humans
- Hybrid Cells
- Kinetics