Article
Systematic mutational analysis of the yeast beta-tubulin gene.
Molecular biology of the cell - 1 Jan 1994
Reijo R A, Cooper E M, Beagle G J, Huffaker T C
Abstract excerpt
A systematic strategy was used to create a synoptic set of mutations that are distributed throughout the single beta-tubulin gene of Saccharomyces cerevisiae. Clusters of charged amino acids were targeted for mutagenesis and converted to alanine to maximize alterations on the protein's surface and minimize alterations that affect protein folding. Of the 55 mutations we constructed, three confer...
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