Article
Systematic mutational analysis of the yeast beta-tubulin gene.
Molecular biology of the cell - 1 Jan 1994
Reijo R A, Cooper E M, Beagle G J, Huffaker T C
Abstract excerpt
A systematic strategy was used to create a synoptic set of mutations that are distributed throughout the single beta-tubulin gene of Saccharomyces cerevisiae. Clusters of charged amino acids were targeted for mutagenesis and converted to alanine to maximize alterations on the protein's surface an...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Benomyl
- Cold Temperature
- Drug Resistance, Microbial
- Fungal Proteins
- Genes, Dominant
- Genes, Fungal
- Genes, Lethal
- Genes, Recessive
- Hot Temperature
- Microtubules
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Protein Folding
- Recombinant Fusion Proteins