Article
Dominant-lethal alpha-tubulin mutants defective in microtubule depolymerization in yeast.
Molecular biology of the cell - 1 Dec 2001
Anders K R, Botstein D
Abstract excerpt
The dynamic instability of microtubules has long been understood to depend on the hydrolysis of GTP bound to beta-tubulin, an event stimulated by polymerization and necessary for depolymerization. Crystallographic studies of tubulin show that GTP is bound by beta-tubulin at the longitudinal dimer-dimer interface and contacts particular alpha-tubulin residues in the next dimer along the protofilament. This...
Topics
- Cell Cycle
- Gene Expression Regulation, Fungal
- Genes, Dominant
- Genes, Lethal
- Green Fluorescent Proteins
- Luminescent Proteins
- Microtubules
- Models, Molecular
- Mutation
- Nocodazole
- Protein Conformation
- Saccharomyces cerevisiae
- Spindle Apparatus
- Structure-Activity Relationship
- Time Factors
- Tubulin