Article
Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha 2-tubulin genes as fission yeast polarity mutants.
Molecular biology of the cell - 1 Jul 1998
Radcliffe P, Hirata D, Childs D, Vardy L, Toda T
Abstract excerpt
We have screened for temperature-sensitive (ts) fission yeast mutants with altered polarity (alp1-15). Genetic analysis indicates that alp2 is allelic to atb2 (one of two alpha-tubulin genes) and alp12 to nda3 (the single beta-tubulin gene). atb2(+) is nonessential, and the ts atb2 mutations we h...
Topics
- Alleles
- Amino Acid Sequence
- Cell Division
- Cell Polarity
- Genes, Fungal
- Genes, Lethal
- Microtubules
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Schizosaccharomyces
- Spindle Apparatus
- Staining and Labeling
- Temperature
- Tubulin