Article
Functional dissection of the gamma-tubulin complex by suppressor analysis of gtb1 and alp4 mutations in Schizosaccharomyces pombe.
Genetics - 1 Jul 2004
Tange Yoshie, Fujita Akiko, Toda Takashi, Niwa Osami
Abstract excerpt
In fission yeast, gamma-tubulin (encoded by the gtb1+ gene), Alp4 (Spc97/GCP2), and Alp6 (Spc98/GCP3) are essential components of the gamma-tubulin complex. We isolated gtb1 mutants as allele-specific suppressors of temperature-sensitive alp4 mutations. Mutation sites in gtb1 mutants and in several alp4 alleles were determined. The majority of substituted amino acids were mapped to a small area on the predicted...
Topics
- Chromatography, Gel
- Cold Temperature
- Immunohistochemistry
- Immunoprecipitation
- Models, Molecular
- Mutation
- Reproduction
- Schizosaccharomyces
- Schizosaccharomyces pombe Proteins
- Sequence Analysis, DNA
- Species Specificity
- Spores
- Suppression, Genetic
- Thiabendazole