Article
STU1, a suppressor of a beta-tubulin mutation, encodes a novel and essential component of the yeast mitotic spindle.
The Journal of cell biology - 1 Dec 1994
Pasqualone D, Huffaker T C
Abstract excerpt
We have isolated a cold-sensitive allele of TUB2, the sole gene encoding beta-tubulin in S. cerevisiae, that confers a specific defect in spindle microtubule function. At 14 degrees C, tub2-406 cells lack a normal bipolar spindle but do assemble functional cytoplasmic microtubules. In an attempt...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Cold Temperature
- Fluorescent Antibody Technique
- Genes, Fungal
- Microtubule-Associated Proteins
- Microtubules
- Molecular Sequence Data
- Mutation
- Protein Binding
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sequence Analysis, DNA
- Spindle Apparatus
- Suppression, Genetic
- Tubulin