Suppression of a conditional mutation in alpha-tubulin by overexpression of two checkpoint genes.

doi:10.1242/jcs.108.3.1195

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Article

Suppression of a conditional mutation in alpha-tubulin by overexpression of two checkpoint genes.

Journal of cell science - 1 Mar 1995

Guénette S, Magendantz M, Solomon F

Abstract excerpt

To identify proteins that regulate microtubule assembly in Saccharomyces cerevisiae, we screened for multicopy suppressors of a conditional mutation in alpha-tubulin. Cells expressing the recessive allele tub1-729 as their sole alpha-tubulin gene grow normally at permissive temperature. However, at 15 degrees C the cells lose viability and arrest primarily with large buds and quantitatively diminished microtubule...

Topics

Alleles
Base Sequence
Cell Cycle Proteins
Cold Temperature
DNA, Fungal
Fluorescent Antibody Technique
Fungal Proteins
Gene Expression
Genes, Fungal
Microtubules
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Phenotype

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Suppression, Genetic

Tubulin

Source

Open in PubMed

Identifiers

PMID: 7622604
DOI: 10.1242/jcs.108.3.1195

Genes

BUB3
BUB1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7622604-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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