Article
Pseudomonas exotoxin A mutants. Replacement of surface-exposed residues in domain III with cysteine residues that can be modified with polyethylene glycol in a site-specific manner.
The Journal of biological chemistry - 6 May 1994
Benhar I, Wang Q C, FitzGerald D, Pastan I
Abstract excerpt
Pseudomonas exotoxin A (PE) is composed of three structural and functional domains. Domain Ia is responsible for cell recognition, domain II for translocation of PE across the cell membrane, and domain III for ADP-ribosylation of elongation factor 2. To investigate the role of the amino acids exp...
Topics
- ADP Ribose Transferases
- Adenosine Diphosphate Ribose
- Animals
- Bacterial Toxins
- Cell Survival
- Cloning, Molecular
- Cysteine
- Escherichia coli
- Exotoxins
- Female
- Mice
- Mice, Inbred BALB C
- Mutation
- Polyethylene Glycols
- Virulence Factors
- Pseudomonas aeruginosa Exotoxin A