Article
Structure-function analysis of exotoxin A proteins with mutations at histidine 426.
Infection and immunity - 1 Mar 1992
Wick M J, Cook J M, Iglewski B H
Abstract excerpt
Substitution of Tyr for His-426 of Pseudomonas aeruginosa exotoxin A results in a mutant protein with reduced ADP-ribosyltransferase activity (M. J. Wick and B. H. Iglewski, J. Bacteriol. 170:5385-5388, 1988). To investigate the role of His-426 in enzymatic activity, oligonucleotide-directed mutagenesis was used to construct mutant proteins encoding Ala, Glu, Gly, Lys, or Pro at position 426. The effect of these...
Topics
- ADP Ribose Transferases
- Bacterial Toxins
- Base Sequence
- Exotoxins
- Histidine
- Molecular Sequence Data
- Mutation
- NAD
- Poly(ADP-ribose) Polymerases
- Protein Conformation
- Pseudomonas aeruginosa