Article
Mutants of immunotoxin anti-Tac(dsFv)-PE38 with variable number of lysine residues as candidates for site-specific chemical modification. 1. Properties of mutant molecules.
Bioconjugate chemistry - 1 Jan 2000
Onda Masanori, Vincent James J, Lee Byungkook, Pastan Ira
Abstract excerpt
Chemical modification of proteins with substances such as poly(ethylene glycol) can add useful properties to proteins. Currently PEGylation is done in a random manner utilizing amino residues dispersed throughout a protein. For proteins such as immunotoxins, which have several different functional domains, random modification leads to inactivation. To determine if we could produce an immunotoxin with a diminished...
Topics
- Amino Acid Sequence
- Disulfides
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Humans
- Immunotoxins
- Lysine
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Oligonucleotides
- Protein Conformation
- Proteins