Article
Pseudomonas exotoxin A mutants. Replacement of surface exposed residues in domain II with cysteine residues that can be modified with polyethylene glycol in a site-specific manner.
The Journal of biological chemistry - 11 Mar 1994
Kuan C T, Wang Q C, Pastan I
Abstract excerpt
Pseudomonas exotoxin A (PE) is a three-domain protein in which domain Ia is involved in recognition of receptors on eukaryotic target cells, domain II promotes translocation of PE into the cytosol, and domain III enzymatically ADP-ribosylates elongation factor 2. Modification of proteins with pol...
Topics
- ADP Ribose Transferases
- Animals
- Bacterial Toxins
- Cells, Cultured
- Cysteine
- Exotoxins
- Humans
- Mice
- Mice, Inbred BALB C
- Mutation
- Polyethylene Glycols
- Protein Folding
- Pseudomonas aeruginosa
- Tumor Cells, Cultured
- Virulence Factors
- Pseudomonas aeruginosa Exotoxin A