An immunotoxin with increased activity and homogeneity produced by reducing the number of lysine residues in recombinant Pseudomonas exotoxin.

doi:10.1021/bc00025a006

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Article

An immunotoxin with increased activity and homogeneity produced by reducing the number of lysine residues in recombinant Pseudomonas exotoxin.

Bioconjugate chemistry - 1 Jan 2000

Debinski W, Pastan I

Abstract excerpt

Pseudomonas exotoxin A (PE) is a protein composed of 613 amino acids arranged into three major, and one minor, domains. Immunotoxins (ITs) containing PE38, a mutant form of PE which lacks the cell binding domain (Ia, amino acids 1-252) and 16 amino acids from domain Ib (amino acids 365-380), are...

Topics

ADP Ribose Transferases
Adenosine Diphosphate
Amino Acid Sequence
Animals
Antineoplastic Agents
Bacterial Toxins
Carcinoma, Squamous Cell
Disulfides
Drug Screening Assays, Antitumor
Escherichia coli
Exotoxins
Female
Humans
Immunotoxins
Lethal Dose 50
Lysine
Mice
Mice, Nude

Source

Open in PubMed

Identifiers

PMID: 8199233
DOI: 10.1021/bc00025a006

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8199233-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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