Article
Analysis of sequences in domain II of Pseudomonas exotoxin A which mediate translocation.
Biochemistry - 23 Jul 1991
Siegall C B, Ogata M, Pastan I, FitzGerald D J
Abstract excerpt
Pseudomonas exotoxin (PE) contains 613 amino acids that are arranged into 3 structural domains. PE exerts its cell-killing effects in a series of steps initiated by binding to the cell surface and internalization into endocytic vesicles. The toxin is then cleaved within domain II near arginine-279, generating a C-terminal 37-kDa fragment that is translocated into the cytosol where it ADP-ribosylates elongation...
Topics
- ADP Ribose Transferases
- Adenosine Diphosphate Ribose
- Amino Acid Sequence
- Bacterial Toxins
- Biological Transport, Active
- Chimera
- Chromosome Deletion
- Electrophoresis, Polyacrylamide Gel
- Exotoxins
- Models, Molecular
- Molecular Sequence Data