Article
Analysis of p53 gene mutations in human gliomas by polymerase chain reaction-based single-strand conformation polymorphism and DNA sequencing.
Diagnostic molecular pathology : the American journal of surgical pathology, part B - 1 Mar 1994
Sarkar F H, Kupsky W J, Li Y W, Sreepathi P
Abstract excerpt
Mutations in the p53 gene have been recognized in brain tumors, and clonal expansion of p53 mutant cells has been shown to be associated with glioma progression. However, studies on the p53 gene have been limited by the need for frozen tissues. We have developed a method utilizing polymerase chain reaction (PCR) for the direct analysis of p53 mutation by single-strand conformation polymorphism (SSCP) and by...
Topics
- Adult
- Base Sequence
- Blotting, Southern
- Brain Neoplasms
- DNA, Neoplasm
- Gene Expression
- Genes, p53
- Glioma
- Humans
- Molecular Sequence Data
- Mutation