Article
Analysis of p53 gene mutations in low- and high-grade astrocytomas by polymerase chain reaction-assisted single-strand conformation polymorphism and immunohistochemistry.
Acta neuropathologica - 1 Jan 1994
Koga H, Zhang S, Kumanishi T, Washiyama K, Ichikawa T, Tanaka R, Mukawa J
Abstract excerpt
Using polymerase chain reaction-assisted single-strand conformation polymorphism (PCR-SSCP) and immunohistochemical analyses, mutations in the p53 tumor suppressor gene were examined in 19 low- and high-grade gliomas. By PCR-SSCP and nucleotide analyses, p53 gene mutation was seen in 7 gliomas. O...
Topics
- Astrocytoma
- Base Sequence
- Brain Neoplasms
- DNA, Neoplasm
- DNA, Single-Stranded
- Glioblastoma
- Humans
- Immunohistochemistry
- Molecular Conformation
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Genetic