Development of a gene cloning system for Streptomyces hygroscopicus subsp. yingchengensis, a producer of three useful antifungal compounds, by elimination of three barriers to DNA transfer.

Abstract excerpt

Streptomyces hygroscopicus 10-22 could not be transformed with any of the commonly used Streptomyces plasmid vectors and was resistant to plaque formation by the Streptomyces phages phi C31 and R4. Repeated selection resulted in the isolation of derivatives of S. hygroscopicus 10-22 that could be transformed with pIJ101- and pJV1-derived cloning vectors and of restriction-deficient derivatives that could accept...

Topics

• Antifungal Agents
• Bacteriophages
• Cloning, Molecular
• DNA Restriction Enzymes
• DNA Transposable Elements
• Gene Transfer Techniques
• Genes, Bacterial
• Genetic Vectors
• Mutation
• Plasmids