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Article

An efficient procedure for marker-free mutagenesis of S. coelicolor by site-specific recombination for secondary metabolite overproduction.

PloS one - 1 Jan 2013

Zhang Bo, Zhang Lin, Dai Ruixue, Yu Meiying, Zhao Guoping, Ding Xiaoming

Abstract excerpt

Streptomyces bacteria are known for producing important natural compounds by secondary metabolism, especially antibiotics with novel biological activities. Functional studies of antibiotic-biosynthesizing gene clusters are generally through homologous genomic recombination by gene-targeting vectors. Here, we present a rapid and efficient method for construction of gene-targeting vectors. This approach is based on...

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PMID: 23409083
DOI: 10.1371/journal.pone.0055906
PMCID: PMC3567011

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Source receipt: pubmed, retrieved 7 Sept 2026.

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