Article
Construction of a genome-editing system for the thermophilic actinomycete Streptomyces thermodiastaticus K5 strain.
Bioscience, biotechnology, and biochemistry - 23 Dec 2024
Yamagishi Kenji, Ike Masakazu, Tokuyasu Ken
Abstract excerpt
Thermophilic actinomycetes significantly contribute to the terrestrial carbon cycle via the rapid degradation of lignocellulosic polysaccharides in composts. In this study, a genome-editing system was constructed for the thermophilic actinomycete Streptomyces thermodiastaticus K5 strain, which was isolated from compost. The genome-editing plasmid (pGEK5) harboring nickase Cas9 was derived from the high-copy...
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