Article
New vector tools with a hygromycin resistance marker for use with opportunistic pathogens.
Molecular biotechnology - 1 May 2011
Kalivoda Eric J, Horzempa Joseph, Stella Nicholas A, Sadaf Aroba, Kowalski Regis P, Nau Gerard J, Shanks Robert M Q
Abstract excerpt
The ability of many bacterial strains to tolerate antibiotics can limit the number of molecular tools available for research of these organisms. To help address this problem, we have modified a diverse set of vectors to include a broadly expressed hygromycin resistance (HmR) marker. Hygromycin B is an aminoglycoside antibiotic not used to treat infections in humans and has antimicrobial activity against a wide...
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