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Directed evolution for thermostabilization of a hygromycin B phosphotransferase from Streptomyces hygroscopicus.

Bioscience, biotechnology, and biochemistry - 1 Jan 2013

Sugimoto Naohisa, Takakura Yasuaki, Shiraki Kentaro, Honda Shinya, Takaya Naoki, Hoshino Takayuki, Nakamura Akira

Abstract excerpt

To obtain a selection marker gene functional in a thermophilic bacterium, Thermus thermophilus, an in vivo-directed evolutionary strategy was conducted on a hygromycin B phosphotransferase gene (hyg) from Streptomyces hygroscopicus. The expression of wild-type hyg in T. thermophilus provided hygromycin B (HygB) resistance up to 60 °C. Through selection of mutants showing HygB resistance at higher temperatures,...

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PMID: 24200799
DOI: 10.1271/bbb.130486

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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