A modified Janus cassette (Sweet Janus) to improve allelic replacement efficiency by high-stringency negative selection in Streptococcus pneumoniae.

doi:10.1371/journal.pone.0100510

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Article

A modified Janus cassette (Sweet Janus) to improve allelic replacement efficiency by high-stringency negative selection in Streptococcus pneumoniae.

PloS one - 1 Jan 2014

Li Yuan, Thompson Claudette M, Lipsitch Marc

Abstract excerpt

The Janus cassette permits marker-free allelic replacement or knockout in streptomycin-resistant Streptococcus pneumoniae (pneumococcus) through sequential positive and negative selection. Spontaneous revertants of Janus can lead to high level of false-positives during negative selection, which necessitate a time-consuming post-selection screening process. We hypothesized that an additional counter-selectable...

Topics

Alleles
Gene Order
Plasmids
Quantitative Trait Loci
Recombination, Genetic
Selection, Genetic
Streptococcus pneumoniae
Transformation, Bacterial

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PMID: 24959661
DOI: 10.1371/journal.pone.0100510
PMCID: PMC4068995

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