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Article

Novel and convenient methods for Candida tropicalis gene disruption using a mutated hygromycin B resistance gene.

Archives of microbiology - 1 Nov 2001

Hara A, Arie M, Kanai T, Matsui T, Matsuda H, Furuhashi K, Ueda M, Tanaka A

Abstract excerpt

We established a novel and convenient method to construct a ura3 strain (ura3/ura3) of the asporogenous and diploid yeast, Candida tropicalis, that produces dicarboxylic acid. One copy of the URA3 gene was disrupted using a mutated hygromycin B resistance gene (HYG#). The obtained hygromycin-resistant strain was further transformed with a URA3 disruption cassette and selected on a plate containing 5-fluoroorotic...

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Source

Identifiers

PMID: 11702078
DOI: 10.1007/s002030100338

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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