Article
New ΦBT1 site-specific integrative vectors with neutral phenotype in Streptomyces.
Applied microbiology and biotechnology - 1 Mar 2016
Gonzalez-Quiñonez Nathaly, López-García María Teresa, Yagüe Paula, Rioseras Beatriz, Pisciotta Annalisa, Alduina Rosa, Manteca Ángel
Abstract excerpt
Integrative plasmids are one of the best options to introduce genes in low copy and in a stable form into bacteria. The ΦC31-derived plasmids constitute the most common integrative vectors used in Streptomyces. They integrate at different positions (attB and pseudo-attB sites) generating different mutations. The less common ΦBT1-derived vectors integrate at the unique attB site localized in the SCO4848 gene (S....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
