Article
[Overcoming restriction of Streptomyces hygroscopicus 10-22 by the modification of S. fradiae--an attempt to develop a transformation system for S. hygroscopicus 10-22].
Yi chuan xue bao = Acta genetica Sinica - 1 Jan 1993
Qin Z, Deng Z, Zhou Q, Chen H
Abstract excerpt
No transformant was obtained when pIJ702 (tsr,mel+) from S. lividans TK24 was used to transform S. hygroscopicus 10-22. pIJ702 isolated from S. fradiae ATCC 10745, however, was transformed into 10-22 at a frequency of 10(3)-10(4) transformants/micrograms DNA. Among the transformant colonies, only...
Topics
- Mutation
- Streptomyces
- Transformation, Bacterial