[Overcoming restriction of Streptomyces hygroscopicus 10-22 by the modification of S. fradiae--an attempt to develop a transformation system for S. hygroscopicus 10-22].

Abstract excerpt

No transformant was obtained when pIJ702 (tsr,mel+) from S. lividans TK24 was used to transform S. hygroscopicus 10-22. pIJ702 isolated from S. fradiae ATCC 10745, however, was transformed into 10-22 at a frequency of 10(3)-10(4) transformants/micrograms DNA. Among the transformant colonies, only 1/1000 of them were black in colour (mel+) while a great majority of them remained white (mel-). Plasraid pIJ702 band...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Mutation
• Share to Streptomyces