Article
Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors.
Nucleic acids research - 25 Feb 1991
Hillemann D, Pühler A, Wohlleben W
Abstract excerpt
For the isolation of single stranded plasmid DNA, various E. coli and E. coli-Streptomyces shuttle plasmids were equipped with the phage f1 replication origin. The transformation of some representative Streptomyces species with plasmid vectors occurred irrespective of whether single or double stranded DNA was used. In contrast, the transformation of Streptomyces was 10 to 100 times more efficient when an...
Topics
- Blotting, Southern
- DNA, Bacterial
- DNA, Single-Stranded
- Escherichia coli
- Genes, Bacterial
- Mutation
- Plasmids
- Streptomyces
- Transformation, Genetic