Transposon Tn5 mutagenesis of pseudomonas fluorescens to isolate mutants deficient in antibacterial activity.

doi:10.1111/j.1574-6968.1994.tb06636.x

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Article

Transposon Tn5 mutagenesis of pseudomonas fluorescens to isolate mutants deficient in antibacterial activity.

FEMS microbiology letters - 15 Jan 1994

Rajendran N, Jahn D, Jayaraman K, Marahiel M A

Abstract excerpt

Pseudomonas fluorescens was subjected to insertion mutagenesis studies using the transposon Tn5-GM to generate mutants deficient in antibacterial activity minus mutants. The transposon located on the temperature-sensitive plasmid pCHR84 was conjugally transferred into the non-pathogenic pseudomon...

Topics

Antibiosis
Chromosomes, Bacterial
Conjugation, Genetic
Crosses, Genetic
Drug Resistance, Microbial
Escherichia coli
Genetic Vectors
Mutagenesis, Insertional
Mutation
Plasmids
Pseudomonas fluorescens

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