Article
Tn5 tagging of the phenol-degrading gene on the chromosome of Pseudomonas putida.
Molecules and cells - 28 Feb 1997
Han E M, Jung Y H, On H Y, Lee M S, Yang Y K, Kim Y, Kim C K, Lee K S, Min K H
Abstract excerpt
Transposon mutagenesis was performed by the method of conjugational transfer in order to identify and characterize genes encoding enzymes involved in the pathway of phenol utilization as a carbon source. Escherichia coli, which carries the Tn5-132, Was mated with Pseudomonas putida SM25 as a host. We selected a mutant that could not utilize phenol as a carbon source. Chromosomal integration of the transposon was...
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