Article
A novel papillation assay for the identification of genes affecting mutation rate in Pseudomonas putida and other pseudomonads.
Mutation research - 1 Aug 2016
Tagel Mari, Tavita Kairi, Hõrak Rita, Kivisaar Maia, Ilves Heili
Abstract excerpt
Formation of microcolonies (papillae) permits easy visual screening of mutational events occurring in single colonies of bacteria. In this study, we have established a novel papillation assay employable in a wide range of pseudomonads including Pseudomonas aeruginosa and Pseudomonas putida for monitoring mutation frequency in distinct colonies. With the aid of this assay, we conducted a genome-wide search for the...
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